U.S. Federal Route 40 runs east-west across much of Washington County. Fro about 40 miles this runs along mostly along surface highway. Near West Brownsville it runs along a expressway. U.S. Rt. 40 passes through the following towns in Washington County. Claysville Lincoln Hill Washington Laboratory Glyde Scenery Hill Beallsville Richeyville West Brownsville U.S. Rt. 40 is multi-plexed in Washington County. Pennsylvania State Route 231, at Claysville. U.S. Rt. 19, from Washington to Laboratory. Pennsylvania State Route 18, at Washington. Pennsylvania State Route 88, near West Brownsville. Pennsylvania State Route 43, near West Brownsville. U.S. Rt. 40 intersects the following highways in Washington County. Pennsylvania State Route 231, at Claysville. Pennsylvania State Route 231, at Claysville. Pennsylvania State Route 221, near Lagonda. Interstate 70, near Lincoln Hill. U.S. Rt. 19, at Washington. Pennsylvania State Route 18, at Washington. Pennsylvania State Route 18, at Washington. Pennsylvania State Route 136, near East Washington. U.S. Rt. 19, at Laboratory. Interstate 79, near Laboratory. Pennsylvania State Route 519, at Glyde. Pennsylvania State Route 917, near Cokeburg. Pennsylvania State Route 418, near Centerville. Pennsylvania State Route 43, near West Brownsville. Pennsylvania Turnpike 43, near West Brownsville. Pennsylvania State Route 88, near West Brownsville. Pennsylvania State Route 88, near West Brownsville.
