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Photosynthesis Lab Procedure Obtain the following materials: Medium test Tube, 1/2 of a spinach leaf, test tube rack. Take the test tube to the back lab counter and measure 2ml of solvent and cut a strip of chromatography paper 15 cm long and take it to your table. Cut the strip in half longways, so that you have 2 strips that are 1 cm wide. Measure up 3 cm from the bottom of each strip and draw a pencil line across. On one strip place the spinach leaf over the penciled line and, with your thumbnail or a coin, rub the pigment out of the leaf onto the strip at the pencil line. Very carefully, insert the strip down into the test tube with solvent, and let the spinach pigments migrate up the strip. Measure the distance that each pigment migrated. You should be able to see: Chlorophyll A- a blue-green line, Chlorophyll B- an olive green line, Carotene- a yellow-orange line, and Xanthophyll-a yellow line. Repeat the process with a black pen or marker in place of spinach on your other strip to see what pigments are in the ink. Remove the strips when the solvent front is about 4 cm from the top of the paper.
1.List the colors you found from the marker/pen chromatogram in order from which they appear from the top to bottom. 2.Measure the distance the individual pigments migrated from the pencil line on both strips. List the pigments and their distances below.
Spinach Marker/Pen Pigment-distance Pigment-distance " "
This information is from the Prentice Hall Biology: The Living World Laboratory Manual made in 1989. |
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