| Team | Nickname | Class | Division | Place in 2000 Champs |
| Bethlehem | Eagles | A | Gold | 4th |
| Burnt Hills-Ballston Lake | Spartans | B | Gold | 8th |
| Colonie | Garnet Raiders | A | Blue | 1st |
| Columbia | Blue Devils | A | Gold | 9th |
| Guilderland | Dutchmen | A | Blue | 5th |
| Mohonasen | Warriors | B | Gold | 10th |
| Niskayuna | Warriors | A | Gold | 7th |
| Saratoga | Blue Streaks | A | Blue | 6th |
| Shaker | Blue Bison | A | Blue | 2nd |
| Shenendehowa | Plainsmen | A | Blue | 3rd |
