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Lab Safety Unit
Mrs. Carol Isler
James F. Byrnes High School
Duncan, SC  29334

 As you view the lab safety video, use the list of major points below to help you take notes in your notebook. Label your notes and divide them into sections that correspond with the sections in the video.

Return this sheet to your teacher when you are finished.  Do not write on it
Summary of Lab Rules
 Handling Chemicals Safely

1. Work with small containers.
2. Mix chemicals only when your teacher says to.
3. Read and reread chemical labels.
4. Labels contain three pieces of information that are important for you to note.
5. Use a work tray if your lab has them.
6. Moves carefully and deliberately when working with chemicals.
7. Add concentrated acids to water.
8. Hold coin top stoppers between your fingers while pouring.
9. Hold bottles with your hand over the label.
10. Work with harmful volatile chemicals under a hood.
11. Keep chemicals as pure and uncontaminated as possible.
12. Draw our chemicals with a pipette filler, never by mouth.
13. Notify your teacher before cleaning up spills.
14. Put waste in the proper container.
15. Clean up when finished.

Bunsen Burner and Glassware Safety

1. Heat volatile organic chemicals in a heating mantle or steam bath in a hood, not over a Bunsen burner.
2. Check the gas hose for cracks.
3. Make the hose fits securely on the gas valve and Bunsen burner.
4. Stand back from the burner while lighting it.
5. Strike matches away from you.
6. Turn on the gas after lighting the match.
7. Turn off the gas immediately of the flame sputters, flares, goes out, or if you smell gas.
8. Check glassware for stars and cracks.
9. Clamp narrow-necked containers to the ring stand.
10. Move test tubes back and forth through the flame at an angle while heating.
11. Hold hot glassware in beaker tongs or hot mits.

Thermometer Safety

1. Don’t shake thermometers.
2. Use thermometers only in the range for which they are suited .
3. Lay thermometers down on a towel or wire screen to cool, away from the edge of the of the bench.
4. Let your teacher clean up broken thermometers.

Glass Tubing Safety

1. Use an inserter to place or remove glass tubing or thermometer in a stopper.
2. Or, lubricate the tubing and protect your hands with leather gloves.

Centrifuge Safety

1. Place equally filled test tubes in a centrfuge to balance it.
2. Don’t try to stop the spinning with your hand.

Behavior in the Laboratory

1. Don’t fool around in lab.
2. Keep aisles clear of personal belongings.
3. Stand on a step stood when you have to reach.
4. Keep makeup in your purse.
5. Keep food and drinks outside.

Dressing for Safety

1. Don’t wear extremely loose clothing.
2. Fabrics should be sturdy and natural.
3. Wear older clothes and cover them with a lab apron.
4. Wear long pants or a long skirt to cover your legs.
5. Wear clothes leather shoes to protect your feet.
6. Tie up long hair.
7. Remove rings and watches.
8. Take out contact lenses.
9. Cover your eyes with goggles with side shields.
10. Protect your hands with the right kind of gloves.

 Emergency Equipment

1. Clean and dry the skin around a cut before a bandage is applied.
2. Rinse chemicals from your eyes in the eyewash fountain.
3. Rinse chemicals from your hands and arms with water from the sink.
4. Remove you clothes on the way to the safety shower to rinse large chemical spills from your body.
5. Extinguish small fires in containers by covering them.
6. Let you teacher use an extinguisher to put out small fires.
7. Put out clothing fires in the safety shower.
8. If there is no other way to put out a clothing fire, use a fire blanket carefully to keep flames away from the face and neck.


Lab Safety Unit Questions

Below are set of questions designed to help you remember some of the most important lab safety practices. Use the  notes you took of the Lab Safety Video to jog your memory. You do not have to copy the questions but you do need to answer them in complete sentences in your notebook.

Handling Chemicals Safely

1. When you read the label on a chemical container, what are the three most important of information?
2. How many times should you read this information before you open the container?
3. Is it important to be exact when preparing a chemical reaction? Explain.
4. What should you do with left over chemicals after an experiment?  What should you do with the products of an experiment?
5. How can you draw small volumes of liquids into a pipette safely?
6. Why is it important to read instructions all the way through before beginning and experiment?
7. When you need to carry chemicals from one place to another in the lab, what size container is the safest to use?
8. If you spill a liquid chemical, what should you do?
9. Should you add acid to water, or water to acid?

Bunsen Burner and Glassware Safety

1. Before hooking a Bunsen burner to the gas line, what should you look for?
2. How quickly should you turn on the gas?
3. When you use a striker, where should you stand?
4. If you flame sputters or goes out, what should you do?
5. If you smell gas in the room, what should you do?
6. Is it safe to heat a sealed container? Explain.
7. Is it safe to work near heated objects? Explain.
8. Is it safe to use glassware that has cracks or stars? Explain.
9. How can you safely carry a heated object?
10. Is it safe to heat flammable chemicals (e.g., gasoline) with a Bunsen burner.

Thermometer Safety

1. Thermometers contain either mercury or alcohol to indicate temperature.  Which of these substances creates toxic vapors when exposed to air?
2. If you break a mercury thermometer, what is the safe way to clean it up?
3. Before choosing a thermometer for a specific job, what should you know?

Glass Tubing Safety

1. What is one of the most common causes of injury in the laboratory?
2. Describe how to use an inserter.
3. If you do not have an inserter, how should you insert glass tubing into a rubber stopper? How can you protedt your hands?
4. What is the safe way to insert a thermometer in a stopper?

Centrifuge Safety

1. If you have one sample that needs to be centrifuged, describe what you must do before turning on the centrifuge?
2. If the centrifuge starts to vibrate or move across the countertop, describe what you should do?
3. How should you stop a spinning centrifuge?
 

Behavior in the Laboratory

1. Is the laboratory a safe place to eat, drink, or put on makeup? Explain why or why not.
2. What is a safe way to read a burette that is above your eye level?
3. Where should you keep personal belongings that you bring to the lab?

Dressing for Safety

1. What types of shoes are appropriate for the lab?
2. Describe the type of clothing you should wear on days when you will be working in the lab?
3. How should you protect your eyes from chemicals and glass shards?
4. Why is it important to remove rings, watches, and contact lenses before working in the lab?
5. How can you protect you hands when working with corrosive chemicals?

Emergency Equipment

1. Name the pieces of safety equipment in the lab. State where they are and how to use them.
2. How do you extinguish a small fire in a container?
3. How do you put out a clothing fire?