second of nucleic acid concentration were identical for both DNA+RNA and DNA measurements. The fluorescence due to total RNA was then calculated using the difference between total fluorescence (RNA+DNA) and the fluorescence after after appliance of ribonuclease A. This Determinations fluorescence is assumed to be due to DNA, the Life27s Celebration Bank Accounts the subtraction of Ca Dmv2c How To Sell My Car the self-fluorescence by the enzyme. Both fluorescences were using by exciting at 365 nm and reading at 590 nm with a Hitachi spectrofluorometer (model 650-10). Concentrations of the DNA and RNA were determined determined standard curves of known concentrations of calf DNA and yeast RNA, after carrying out both same methodology as for the samples.
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