N.C.A.A. Division III National Champions
Women's Outdoor 400-Meter Dash
| Year | Name | School | Performance |
| 2011 | Nevada Morrison | Wartburg (IA) | 54.74 |
| 2010 | Rachel Boerner | Calvin (MI) | 54.50 |
| 2009 | Liz Kooistra | Wisconsin-Eau Claire | 54.29 |
| 2008 | Rachel Anderson | Illinois Wesleyan | 54.35 |
| 2007 | Amanda Donath | Wisconsin-Oshkosh | 54.68 |
| 2006 | Rachel Anderson | Illinois Wesleyan | 54.98 |
| 2005 | Tiffany Clark | College of New Jersey | 54.59 |
| 2004 | Amber James | Wheaton (MA) | 53.58 |
| 2003 | Amber James | Wheaton (MA) | 54.25 |
| 2002 | Amber James | Wheaton (MA) | 53.81 |
| 2001 | Amber James | Wheaton (MA) | 54.20 |
| 2000 | Megan Pierce | Calvin (MI) | 53.97 |
| 1999 | Megan Pierce | Calvin (MI) | 54.17 |
| 1998 | Jennifer Culley | Claremont-Mudd-Scripps (CA) | 54.11 |
| 1997 | Jacquiline Hunter | City College of NY | 54.90 |
| 1996 | Lisa Melendez | Wisconsin-Oshkosh | 55.56 |
| 1995 | Lisa Melendez | Wisconsin-Oshkosh | 56.43 |
| 1994 | Patsy Cargill | North Central (IL) | 55.10 |
| 1993 | Kelli Sheda | Simpson (IA) | 55.25 |
| 1992 | Jacqui Dent | Occidental (CA) | 54.26 |
| 1991 | Jacqui Dent | Occidental (CA) | 54.95 |
| 1990 | Vera Stenhouse | Tufts (MA) | 55.89 |
| 1989 | Carolyn Ross | Augsburg (MN) | 54.84 |
| 1988 | Genesia Eddins | Mass.-Boston | 53.45 |
| 1987 | Genesia Eddins | Mass.-Boston | 54.25 |
| 1986 | Trena Marshall | City College of NY | 55.02 |
| 1985 | Genesia Eddins | Mass.-Boston | 55.34 |
| 1984 | Nancy Cisar | Simpson (IA) | 55.79 |
| 1983 | Natalie Jordan | Willaim Paterson (NJ) | 54.73 |
| 1982 | Melissa Hill | Frostburg State (MD) | 56.28 |
