N.C.A.A. Division I National Champions
Women's Outdoor 400-Meter Dash
| Year | Name | School | Performance |
| 2011 | Jessica Beard | Texas A&M | 51.10 |
| 2010 | Francena McCorory | Hampton (VA) | 50.69 |
| 2009 | Joanna Atkins | Auburn | 50.39 |
| 2008 | Shana Cox | Penn State | 50.97 |
| 2007 | Natasha Hastings | South Carolina | 50.15 |
| 2006 | Clora Williams | Texas A&M | 51.11 |
| 2005 | Monique Henderson | UCLA | 50.10 |
| 2004 | Dee Dee Trotter | Tennessee | 50.32 |
| 2003 | Sanya Richards | Texas | 50.58 |
| 2002 | Allison Beckford | Rice | 50.83 |
| 2001 | Allison Beckford | Rice | 52.33 |
| 2000 | Mikele Barber | South Carolina | 51.14 |
| 1999 | Suziann Reid | Texas | 51.08 |
| 1998 | Suziann Reid | Texas | 51.22 |
| 1997 | LaTarsha Stroman | Louisiana State | 50.60 |
| 1996 | Suziann Reid | Texas | 52.16 |
| 1995 | Nicole Green | Kansas State | 52.01 |
| 1994 | Flirtisha Harris | Seton Hall (NJ) | 51.65 |
| 1993 | Juliet Campbell | Auburn | 50.58 |
| 1992 | Anita Howard | Florida | 51.01 |
| 1991 | Ximeno Restrepo | Nebraska | 51.01 |
| 1990 | Maicel Malone | Arizona | 51.13 |
| 1989 | Pauline Davis | Alabama | 50.18 |
| 1988 | Rochelle Stevens | Morgan State (MD) | 51.23 |
| 1987 | Lillie Leatherwood-King | Alabama | 50.90 |
| 1986 | Lillie Leatherwood | Alabama | 51.23 |
| 1985 | Sherri Howard | Cal State-Los Angeles | 50.95 |
| 1984 | Marita Payne | Florida State | 51.05 |
| 1983 | Florence Griffith | UCLA | 50.94 |
| 1982 | Marita Payne | Florida State | 52.01 |
