N.A.I.A. National Champions
Women's Outdoor 400-Meter Hurdles
| Year | Name | School | Performance |
| 2011 | Nikita Tracey | Oklahoma Baptist | 58.11 |
| 2010 | Verone Chambers | Oklahoma Baptist | 58.60 |
| 2009 | Verone Chambers | Oklahoma Baptist | 58.80 |
| 2008 | Sherene Pinnock | Oklahoma Baptist | 59.50 |
| 2007 | Lorraine Swaby | Azusa Pacific (CA) | 59.81 |
| 2006 | Nickiesha Wilson | Oklahoma Baptist | 58.25 |
| 2005 | Shawna Kern | Mary (ND) | 60.08 |
| 2004 | Anabell Montes | Warner Southern (FL) | 59.07 |
| 2003 | Anabell Montes | Warner Southern (FL) | 61.04 |
| 2002 | Kendra Doyle | Bethel (KS) | 61.23 |
| 2001 | Natalie Jackson | Simon Fraser (BC) | 60.02 |
| 2000 | Sedell Roberts | Life (GA) | 59.02 |
| 1999 | Neisha Thompson | McKendree (IL) | 60.45 |
| 1998 | Jamey Mulske | Mary (ND) | 61.32 |
| 1997 | Lesakay Morgoridge | Southern-New Orleans | 59.63 |
| 1996 | Catherine Pomales | Central State (OH) | 56.98 |
| 1995 | Catherine Pomales | Central State (OH) | 58.04 |
| 1994 | Catherine Pomales | Central State (OH) | 56.57 |
| 1993 | Deon Hemmings | Central State (OH) | 55.14 |
| 1992 | Deon Hemmings | Central State (OH) | 56.56 |
| 1991 | Deon Hemmings | Central State (OH) | 58.14 |
| 1990 | Wendi Simmons | Graceland (IA) | 60.42 |
| 1989 | Wendi Simmons | Graceland (IA) | 61.06 |
| 1988 | Susanne Johansson | Point Loma Nazarene (CA) | 59.25 |
| 1987 | LaVonda Luckett | Prairie View A&M (TX) | 57.39 |
| 1986 | LaVonda Luckett | Prairie Veiw A&M (TX) | 58.09 |
| 1985 | LaVonda Luckett | Prairie View A&M (TX) | 58.89 |
| 1984 | LaVonda Luckett | Prairie View A&M (TX) | 60.78 |
| 1983 | Margaret Gamble | Prairie View A&M (TX) | 58.99 |
| 1982 | Margaret Gamble | Prairie View A&M (TX) | 59.30 |
| 1981 | Felicia Durham | Southern-Baton Rouge | 60.47 |
