!Help file for !Colocal v0.2 16/10/2002 - Freeware
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This is an early version of an app for use with immunofluorescence confocal microscope images. On a slide where two antibodies with different fluorophores have been used colocalisation can be looked for by combining the two images into one. This app takes two sprites (e.g. red image and green image) and merges them. 

How to use
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Doublie-click to load !Colocal and then click on the iconbar icon. A window will open. Simple drag (one at a time) the two sprites to be combined onto the two icons. Then click OK.

When the merged sprite is displayed on the screen, pressing S will save the sprite and pressing Q will return to the desktop. Any other key toggles between image one and the merged image so the change can be easily seen.

Notes:

* The saved sprite is called 'merged' and is saved inside the !Colocal app (Shift double click !Colocal to open it)

* The mode used for display is 640x512 in 32 thousand colours (32k). 
  This is for two reasons:
  1) All my confocal images are 512x512 so this mode shows them at their largest
  2) I only have 1MB VRAM so can't do 16 million colours (16M) in this mode

As a consequence any sprites dragged to the window must be 32k/15bit sprites and not 16M/24bit sprites. If you want to use 16M/24bit sprites and have enough VRAM, just change the first line of the BASIC program 'merge' found in the !Colocal app directory.

mattlb@angelfire.com