Instructions: Except
as noted, the following schedule of laboratory exercises can be found in
the Lab Manual : "Selected Exercises from Microbes in Action by Seeley and Van
Demark.
Students should read each
exercise before coming to the lab. There may be a pre-lab quiz before the lab.
The lab schedule is tentative and may be modified
Date
9-5
1.
Experiments 5 and 6 (Asceptic Transfer of Bacteria into broth and agar slants)
1.
Use of the Microscope
1.
Experiment 1 (Examination of stained cells under oil immersion).
9-9
2. Experiment 10: Direct Staining with Basic Dyes
2.
Handout will be provided for Negative Staining.
(students will stain cultures they prepared in
experiments 5 and 6)
9-11
3.
Experiment 11: The Gram Stain (Differentiating between bacterial types)
For
help interpreting Gram Stains Go to:
http://www.meddean.luc.edu/lumen/DeptWebs/microbio/med/gram/exam-gs.htm
3. Experiment 12:(Only Endospore Stain part).
9-16
4.
Experiment 27: Carbohydrate Degradation,
https://www.angelfire.com/mi/nccc/sugar.html
Click here to see lab results from last years Carbohydrate
4. Handout on Lipid Hydrolysis
9-18
5. Experiment 28: Degradation of Proteins and Amino Acids
https://www.angelfire.com/mi/nccc/milk.html
Click here to see an example of milk reactions from
9-23
6.
Experiment 17: Osmotic pressure lab handout
https://www.angelfire.com/mi/nccc/product.html
Click here to see the effect of salt on bacterial
6. Experiment 30: Catalase Activity -Parts A and B.
9-25
7.
Experiment 7: Separating Mixed Cultures into Pure Cultures.
https://www.angelfire.com/mi/nccc/Separate.html
Click on this line to view lab results from last
7. Experiment 14: Quantitative Plating Methods: Determination of Microbial Numbers: Pour Plate method. (p. 84 in Manual. Read preceding pages).
9-30
8.
Experiment 19 Energy Sources and Buffers
8.
Experiment 32 (modified) Selective plating EMB
https://www.angelfire.com/mi/nccc/labwork.html
Click here to learn how to tell the difference between
8.
Also Acid-fast and capsule staining
10-2
9.
Experiments 17 Effects of Temperature
9.
Experiments 18 Effects of Heat
9. Experiments 21 and 22: Oxygen and Microbial Growth and Culture of Anaerobic Bacteria
10-7
10.
Experiment 9(demonstration only)
10.
Review unknown procedure
10. Notebooks Due
10-9
11.
Chapter 11/Distribute Unknowns.
10-14
12. Chapter 12/Unknowns.
10-16
13.
Experiment 23 and Fingerprint plates(handout)/Unknowns
10-21
14. Unknowns.
10-23
15. Experiment 51 Antibiotics/Unknowns.
10-28
16
Unknowns write-up due/ UV light
experiment Handout
16. LAb test #1/Notebooks due
10-30
17. Transformation Experiment (Handout)
11-4
18.
Plasmid Prep(Handout)
11-6
19.
Plasmid Identification(Handout)
11-11
20.
Plasmid Identification(continued).
20. HIV Simulation Handout.
11-13
21. Discuss Group Lab projects
11-18
22.
An Antibody ID Test: Staph Latex Test .
22. ELISA (Handout)
11-20
23.
Experiment 40 Coliform Standard Analysis of Local Water
11-25
24. Experiment 40 continued.
12-2
25.
Lab Test #2/Notebooks due
12-4
24-27
Work on Lab Projects
12-9
25-27.
Lecture Presentations(first 50 minutes)
12-11
28.
Present Lab Projects
Grading:
Notebooks/Handouts: 30%
Pre-lab quizzes: 10%
Tests: 20%
Unknowns: 20%
Group Projects: 20%