Hair is an important feature of mammals, along with human evolution, hair (mainly refers to the hair) in human social life has become external representation of health and beauty, and its social and psychological significance far beyond their physiological significance . Hair condition can significantly affect the patient's self-evaluation, social relations and quality of life, supple, shiny and healthy hair and beautiful hairstyles often give people an advantage in social activities. Alopecia clinical symptoms were more common, often to patients with psychological stress and inconvenience of life, therefore, baldness has become an increasingly modern people concern. Alopecia in clinical classification: seborrheic alopecia, neurological alopecia, scarring alopecia, alopecia disease, hereditary alopecia, alopecia acquired. Alopecia causes are many and complex, there are: mental or physical factors, androgen alopecia, drug factors, food and nutritional and metabolic factors, congenital factors, physical and chemical factors, endocrine disorders factors , the reaction function of the immune system disorder that and other reasons. Among the many causes of baldness, especially androgenic alopecia caused the highest incidence of alopecia, the most common. Clinical treatments for baldness include: drug therapy, surgery, beauty treatments and use of various techniques alopecia conceal hidden beauty treatments. Drug treatment: the treatment of alopecia effective drugs commonly used in two ways: m if to Seoul (Regaine ninoxidil) and finasteride (Propecia finasteride). Regaine minoxidil hair papilla cells can upregulate vascular endothelial growth factor mRNA expression, whereas dermal papilla cells of vascular endothelial growth factor on hair growth cycle has an important regulatory role; through the cytokine stimulation, to maintain and extend the hair follicles growing hair follicle growth , the slight increase of the hair follicle dermal papilla and promoting angiogenesis, thus play an important role in the treatment of hair loss. Propecia fnasteride reduction for 5-α isozyme inhibited, thereby inhibiting testosterone reduced to dihydrotestosterone (Dihydrotestosterone, DHT), decreased serum concentration of DHT in the scalp and male hormones would not be restored in the 5-α isoenzyme The role of inhibition of hair growth down, thereby effectively reversing the process of alopecia, alopecia and promote hair re-growth area. Using these two drugs can effectively prevent the various causes of hair loss, baldness or thinning hair has been promoting hair regrowth sites, but require prolonged use, weight and hair Hair Extensions after disabling the declining trend in the number of levels, suggesting that drugs are not able fundamentally change the patient's genetic predisposition hair loss, and will bring hirsutism, decreased libido and sexual dysfunction and other side effects. Surgical treatment: in 1939, Dr. Okuda of Japan to take technology as a Punch in patients with severe burns hair transplant surgery, the patient made a round of the occipital skin tissue with hair, resulting in the transplant site grow new hair, opened a modern new chapter in hair transplantation surgical techniques, the development has been nearly 70 years of history. Clinically for the treatment of alopecia surgical techniques include the following methods: flap surgery, scalp reduction surgery, scalp tissue expansion, scalp autologous transplantation technology, but because of the presence of the donor site scar and hair transplant morphology uneven situation, and surgery long cycle, affecting the lives of patients and work issues such as the above method fails to achieve satisfactory results. In the late 1990s, Canada and the United States, Dr. Seager and Dr. Rassman proposed "the concept of follicular units" and "microscopic follicular unit grafting" technology, so autologous hair follicle transplant technology has been widely promoted. This migration technology not only greatly improve the survival rate of transplanted hair, and the hair transplant morphology is also more natural, and its significant and lasting results generally welcomed by patients. Requirements of autologous hair follicle transplantation patients must have sufficient donor hair follicles, but for alopecia area is too large, or even complete absence of hair scarring alopecia patients generally sparse or full head of hair with top rare female androgenetic alopecia patients, because the donor or recipient sites in poor condition, inadequate sources of hair follicles, hair follicle survival rate after transplantation into the recipient area is unstable, after the hair density and appearance can not achieve satisfactory results. So, how to solve the apparent lack of resources for the District of hair, hair restoration surgery clinical become urgent today to explore the issue. Beauty Technology: alopecia patients if mental suffering, mental burden, as well as the treatment gradually lost confidence, will inevitably lead to the aggravation of the symptoms, in this case, the patient can choose to wear a wig or hair weave, reduce the mental burden, to accept drugs or Surgical treatment is combined in order to obtain better results. While weaving hair wigs or alopecia have some cover cosmetic effect, to give patients a certain psychological comfort, but wear wigs exist three drawbacks, one of which is the structure is not strong, easy to fall off by external forces; secondly wear the head feels hot, tight, and the short period of use: its three wig hair easy to mess, easy out, it will end plate, combing inconvenience unnatural appearance, beauty treatments therefore not fundamentally ease the patient's psychological pressure. Domestic and foreign research scholars tried experimental study to explore new ways to treat baldness disease. The hair hunman hair wigs follicle tissue engineering currently being rebuilt by in vitro follicle, hair follicles allogeneic transplantation and gene therapy in these areas to seek a breakthrough. Carried out by means of tissue engineering autologous hair follicle cells cultured in vitro amplification of its quantity, laid the theoretical experimental basis. Abroad has been carried out in the laboratory and transplanted follicle cells allogeneic success story: Researchers healthy adult human hair follicle epithelial cells transplanted into another forearms of healthy adults showed that caused the epithelial-mesenchymal reaction to produce the new hair follicle cells and grow. However, these studies are still stuck in the experimental stage, the distance is still a long road to clinical application. In summary, at home and abroad for autologous lack of resources for the District of hair alopecia patients, regardless of clinical drug treatment, surgical treatment, cosmetic treatment, or basic research laboratory, at this stage have not been able to find a practical and satisfactory treatment. Yi Bin, Hu Zhiqi other experimentally explore a portable wig, and obtained national patent. The wig structure includes a cylindrical polypropylene barbed with anchor base, top with a hair firmly connected, obviously inadequate resources for the treatment of alopecia hair provides a new way of thinking. But the shortcomings of the wig is: Since each portable wig and a hair bonding only after transplantation, the appearance of thinning hair, not natural, simulation results are poor. In this study, the structure of the graft modified wig, and through animal experiments to observe the tissue compatibility and appearance simulation results, and explore the possibility of its use for clinical, to address the autologous donor hair baldness problem of insufficient resources to provide a more A new method of clinical utility. One, portable wig structure improved design and production Objective: In this study, our group portability wig on the basis of a preliminary study to explore the structure of portability wig improved method and design the best structure for subsequent animals experimental basis. Method: 1, portability wig base production: the polypropylene powder is injected into a diameter of 1mm, made of stainless steel cylinder mold, the mold is placed in the experimental Digital Blast oven, without changing the chemical properties of the polypropylene under the premise of a polypropylene powder inside the mold in the heating conditions of the glass transition viscous flow state, and the formation of the mold corresponding to a cylinder diameter of 1mm. 2, the base of the hair fiber bundle convergence: by 8 real hair fibers is 5.0cm long hair bundles insert mold, the roots of the hair bundle is still in the viscous flow state with polypropylene solid fusion hair bundle buried in polypropylene within the base. At room temperature, forming a cylindrical mold a glass transition polypropylene base, to mold, to form the hair fiber bundle with a polypropylene base. In binocular stereo dissecting microscope, using the 11th blade wig graft polypropylene base anchor carve multiple serrated spines, from the appearance of visible wig graft polypropylene base surface showing multiple rows jagged. Conclusions: 1, polypropylene-based materials, wig, a good tissue compatibility, is clinically proven to be retained in the body for long-term medical polymer materials, to ensure clinical safety. 2, improved portability wig graft can be more firmly anchored in the subcutaneous tissue, and polypropylene base increase in the number of convergence hair, get a more natural look. 3, in order to lay the foundation for the subsequent animal experiments. Second, portability wig rabbits implanted subcutaneous tissue compatibility and appearance Observation Objective: The structure of the improved portability wig rabbits subcutaneous implants, and through animal experiments and observe its histocompatibility Portable wig appearance simulation results, explore its possibilities for clinical. Methods: 10 New Zealand white rabbits (Southern Medical University Experimental Animal Center), in each rabbit subcutaneous use of follicular unit transplantation, implantation of 40 bundles wig grafts, including eight hairs lace wigs per bundle, 10 white Rabbits were transplanted 400 bundle. The 10 rabbits were randomly divided into two groups, namely general observation group and histological groups of five. Gross observation group MAIN OUTCOME 1 year graft shedding and counting; histology group were the first week after transplantation, the first 2 weeks and 1 year after the month under sterile conditions, each rabbit fractionated Collection includes four beams full-thickness skin graft tissue pieces, HE staining optical microscopy and scanning electron microscopy histological observation. Conclusions: 1. Improved graft wig can be firmly anchored in rabbit skin, graft polypropylene base and rabbit subcutaneous tissue biocompatibility, no see adverse reactions. (2) improved graft wig more natural in appearance with the simulation results. 3. Explore solutions to the clinical problem of insufficient donor source follicles. (4) The experiments were carried out for one anniversary vivo experimental studies, confirmed that the portability wig clinical utility and safety, clinical trial for the subsequent experiments laid the theoretical foundation.
