Katsuragi Misato, 29 years old. She belongs to the first section of military operation bureau of the tactical military operations department of NERV. She hates Angels very much and took destroy Angels as her responsible. She is cool, humour but a little bit lazy. Her only best friends in NERV is Ritsuko. She likes to drink beer and eats instant noodles by mixing a lot of curry in it. She never did the housework. Before Shinji came to live with her, her house is a mess. She later becomes the guardian of Shinji and Asuka after they move to stay with her. Shinji also complains about her laziness and not dressing well in house to Touji and Kensuke. She once lived with her ex-boyfriend, Kaji Ryouji, but they have separated long time ago.

Katsuragi Misato is the only survivor in the Katsuragi Expedition which is organized by her father in order to look for Adam described in Dead Sea Scrolls. Her father only cared about his experiment and ignored his family. Misato's mother was very sad and Misato hated him very much. But before the Second Impact occurred, her father sacrificed himself in order to save her from death. After that, she suffered from a kind of mental illness and didn't talk any words for almost five years. Nine years after the Second Impact, she enters and becomes the first section of military operation bureau of the tactical military operations department of NERV.