Magnification: Low

Explanatory notes:

Satisfactory squamous cellularity on a

 ThinPrep slide: An adequate liquid based

 preparation should have an estimated

 minimum of 5,000 well-visualized/preserved

 squamous cells. Endocervical cells are also

 present.

Magnification: High

Explanatory notes:

Satisfactory squamous cellularity. NILM (Atrophy):

Liquid based preparations with atrophy show less nuclear enlargement than conventional smears. It is due to improved fixation and cleaner background than in conventional smears. An adequate liquid based preparation should have an estimated minimum of 5,000 well-visualized/preserved squamous cells.

Magnification: Low

Explanatory Notes:

Unsatisfactory: scant squamous cellularity:

This specimen is unsatisfactory due to scant squamous cellularity seen at 10X.

An adequate liquid based preparation should have an estimated minimum of 5,000 well-visualized/preserved squamous cells.

Magnification: Low

Explanatory Notes:

Unsatisfactory due to scant squamous cellularity: Groups of endocervical cells are seen as well as debris.

An adequate liquid based preparation should have an estimated minimum of 5,000 well-visualized/preserved squamous cells.

Magnification: High

It is essential to identify one or more of the following:

nucleus, eosinophilic granules, flagella, in order to distinguish trichomonads from cytoplasmic fragments

Magnification: High

Cytomorphologic Criteria:

In this image are numerous trichomonads adhering to a squamous cell.

Trichomonas is a pear-shaped, oval to round, cyanophilic organism. It ranges in size from 15-30 microns. The nucleus is pale, vesicular and centrally located. Eosinophilic granules are often visible in the cytoplasm.

Magnification: High

Explanatory Notes:

Pseudohyphae formed by elongated budding

 yeast showing constrictions along their

 length. Pseudohyphae and reactive changes

 in the squamous epithelial cells

Magnification: High

Small, uniform, round budding yeast.

Candida: Pseudohyphae, formed by

elongated budding, with spearing of epithelial

cells--"shish kebob" effect.
　

Magnification: High

Individual squamous cells covered by a layer

of bacteria that obscures the cell

 membrane, this is  the clue cell.

Magnification: Medium

Filmy background of small coccobacilli.

Individual squamous cells covered by a layer

of  bacteria. Conspicuous absence of

lactobacilli.
　

Magnification: Medium

Explanatory Notes:

Actinomyces is often associated with intrauterine device (IUD) usage. Organisms may alert clinician to look for evidence of pelvic infection.

Cytomorphogically actinomyces appear as tangled clumps of filamentous organisms, often with acute angle branching, sometimes showing irregular wooly appearance. Swollen filaments may be seen with clubs at periphery.

Magnification: High

The center of this sulfur granule shows amorphous

 material, while the periphery exhibits thin

 filamentous bacilli radiating outwards.
　

Magnification: Medium

Explanatory Notes:

Nuclei with "ground-glass" features are the most common change identified with Herpes.

The ground-glass appearance of the nuclei is due to accumulation of viral particles leading to peripheral margination of chromatin.

Dense intranuclear inclusions which are often eosinophilic, surrounded by a halo or clear zone.

Magnification: High

"Ground-glass" appearance is due to intranuclear

 viral particles and enhancement of nuclear

 envelope caused by peripheral chromatin

 margination.

Magnification: High

Explanatory notes:

Background shows granular debris which

can mimic tumor diathesis, i.e. by 'clinging'

to cell groups. Parabasal cells with some

nuclear degeneration.

Nuclei are usually smaller with less air

drying artifact.

Magnification: High

Explanatory notes:

Nuclei are uniformly large, and contain prominent

 nucleoli. Chromatin abnormalities are absent. The

 cell cluster shows inter-cellular windows i.e. school-

of-fish appearance and a streaming pattern.

Magnification: High

Parabasal cells which resemble metaplastic cells in atrophy. Generalized nuclear enlargement is present in parabasal cells but without significant hyperchromasia.

Nuclei are present in a sheet with a microbiopsy appearance. they show fine chromatin and appear uniform and bland.

Magnification: High

Atrophic vaginitis: atrophy with inflammation.

Background shows granular debris which can mimic tumor diathesis.
Parabasal cells with some nuclear degeneration.

Magnification: High

Small, round nuclei similar in size to a normal intermediate cell nucleus. Cells occur in small clusters.  Cells borders are ill-defined. Inconspicuous or absent nucleoli. Cytoplasm is scant, basophilic and sometimes vacuolated.
Endometrial cells after age 40, particularly out of phase or after menopause may be associated with benign endometrium, hormonal alterations and less commonly, endometrial /uterine abnormalities. Clinical correlation is recommended.
　

Magnification: High

The endometrial cell nuclei are the same size as the intermediate squamous cell nucleus. A single vacuolaed cell at group edge.
Endometrial glandular group "above" the squamous cell plane of focus. Apoptotic cell centrally.