Dr. El-Sherif, Ph.D.
College of Staten Island
Staten Island, NY 10314
1. 1998 – Present CUNY Graduate School
PhD. in Neuroscience (12/2002)
2. 2001 CUNY Graduate School
Master of Philosophy (Biology)
3. 1994 – 1998 College of Staten Island
1. Fall 2002 College of Staten Island
Anatomy & Physiology (Biology 150 – Lecture and Lab)
2. Spring 2001 College of Staten Island
Introduction to Human Anatomy & Physiology (Biology 102 - Lab)
3. Fall 2000 College of Staten Island
Anatomy & Physiology (Biology 150 - Lecture)
4. Spring 2000 College of Staten Island
Biology for Non-Science Majors (Biology 107 - Lab)
1. 2001-Current Research Foundation
Assist in research projects (unrelated to my dissertation), as well as maintain and
upgrade electrophysiological equipment.
2. 1998 – 1999 College of Staten Island
College Assistant (Animal Caretaker)
Care of animals in the science buildings animal facility (at the College of Staten
Island). Handling of mice, rats, pigeons and canaries.
3. Fall 1998 College of Staten Island
CLT for CHM 250
Prepared organic chemistry labs for undergraduate chemistry course.
4. 1996 – 1997 Brooklyn V.A. Medical Center
Worked at the Brooklyn V.A. medical center assisting in clinical cardiology based
research (development of new Holter monitor which utilizes a computer based flash
memory card to store signals for off-line analysis).
1. Yasir El-Sherif, Neil Singh, Tara Khan and Andrzej Wieraszko "The effects of glutamate uptake inhibitors on hippocampal evoked potential in vitro" Acta Neurobiolo. Experimentalis (1999) 59(2): 89-97.
2. Tatyana Adayev, Yasir El-Sherif, Madhabi Barua, Nicholas J. Penington and Probal Banerjee "Agonist Stimulation of the Serotonin1A Receptor Causes Supression of Anoxia Induced Apoptosis via Mitogen-Activated Protein Kinase in Neuronal HN2-5 Cells" J. Neurochem. (1999) 72: 1489-1496.
3. Luiz G.F. Lopes, Andrzej Wieraszko, Yasir El-Sherif, Michael J. Clarke "The trans-labilization of nitric oxide in RuII complexes by C-bound imidazoles" Inorganica Chimica Acta (2001) 312: 15-22.
4. Michael Hogan, Yasir El-Sherif, Andrzej Wieraszko "The Effects of Melatonin on Hippocampal Evoked Potentials" J. Pineal Res. (2001) 30(2): 87-96.
5. Yasir El-Sherif, Andrzej Wieraszko, Probal Banerjee and Nicholas J. Penington “ATP induces a non-selective cation current and modulates Na channel gating in a hippocampal neuronal cell line” Brain Research (2001) 904(2): 306-317.
6. Yasir El-Sherif, Michael V. Hogam, Jene’ Tesoriero, Andrzej Wieraszko “Factors Regulating the Influence of Melatonin on Hippocampal Evoked Potentials: Comparitive Studies on Different Strains of Mice.” Brain Research (2002) 945(2): 191-201.
1. Hogan, M., Y. El-Sherif, A. Wieraszko. Melatonin modulates the neuronal activity of the mouse hippocampus. Society for Neuroscience Abstract (2000) 30:76.25
2. Wieraszko, A., Hogan, M., El-Sherif, Y., Tesoriero, J., Raja, H. The influence of melatonin on the hippocampal glutamergic system. Journal of Neurochemistry Abstracts (2002) 81(supl. 1): AP11-09.
1. Reviewed a textbook for Benjamin Cummings titled, “Principles of Human Physiology, by William J. Germann and Cindy L. Stanfield” © 2002
2. Received 3 continuous years of radiation training and licensing.
1. Tissue culture – culturing neuroblastomas (HN2, F-11, etc.)
2. Preparation of mouse hippocampal slices.
3. Extracellular field recordings from hippocampal slices (Population Spike, EPSP and Fiber Volley; refer to El-Sherif et al. 1999, Luiz et al., 2001, Hogan et al., 2001, El-Sherif et al., 2002). Studies in the hippocampus including LTP (induced by High frequency Stimulation - HFS), paired pulse inhibition, paired pulse facilitation (manuscript in review).
4. Extracellular field recording from canary slices (recorded from RA nucleus and HvC).
5. Whole cell patch-clamp technique (refer to Adayev, et al, 1999 and El-Sherif et al., 2001). Recorded Voltage-gated Na+ and Ca2+ channels from a number of different cell lines, including HN2, HN2-5, F-11, and acute hippocampal neurons.
6. Radioligand binding – [3H]-Melatonin Binding (El-Sherif, 2002) and [3H]-Glutamate Binding using both a modified centrifugation technique and a rapid filtration technique.
7. [3H]-D-aspartate uptake and release from crude hippocampal synaptosomes. Designed a simple system to perfuse hippocampal synaptosomes and measure release. More recently began using Brandel Suprafusion 1000 system for release studies.
8. General use of Confocal laser scanning microscope (using both an older Leica system and newer Olympus system). Utilized in the imaging of: a) GFAP labeled astrocytes from cerebellum and, b) calcein AM and propidium iodide double stained sections from both mouse and pigeon hippocampus.
9. Intracellular injection of fluorescent dyes (Luciferase) into hippocampal neuroblastomas.
Current Research Projects:
1. We are also currently testing different kinase inhibitors, for their ability to modulate the attenuation and/or the recovery/amplification phases of melatonin.
2. In another set of experiments we are looking at the: a) interaction of melatonin with glutamate binding to hippocampal synaptic membranes and b) the effect of melatonin on uptake/release of [H3]-D-Aspartate from crude hippocampal synaptosomes.
3. Experiments are also being performed to characterize the electrophysiological responses of hippocampal slices from fragile X mice (Fmr-1).
4. Studies are also underway to identify voltage-gated Ca2+ channel expression in hippocampal cell lines, following transfection of an unrelated gene (manuscript in review).