Site hosted by Angelfire.com: Build your free website today!


John W. Semple's Laboratory Homepage

Updated March 9, 2009
Disclaimer: Please note that Dr. Semple does not endorse nor advocate the use of any products advertized by Angelfire on this site. The only therapies that are "proven" to help patients with ITP are those that have undergone the scutiny of prospective randomized controlled clinical trials. Herbal formalas and many other products HAVE NOT been "proven" to help patients with ITP. Anyone that sells them with claims that they help or cure ITP are simply trying to get your money. PLEASE BEWARE.




Welcome.


Let me introduce myself.

I am a Senior Scientists at St. Michael's Hospital and a Professor at the University of Toronto in Toronto, Canada.




I am also a member of the Toronto Platelet Immunobiology Group (affectionately known as TPIG). Its a network of scientists and physcians which are committed to transfusion immunology research. We are also active in disseminating information about immune blood cell disorders. The group has close links with the Hospital for Sick Children, and the Canadian Blood Services.

The current members of my laboratory are Ed Speck, Michael Kim, Rukhsana Aslam and Leola Chow.


Our research is in the area of platelet immunology. We primarily study the pathogenic immune mechanisms responsible for autoimmune (e.g. ITP or AITP) and alloimmune (e.g. transfusion-induced) platelet disorders and the mechanism of action of established clinically "proven" therapies such as IVIg and anti-D. Related to these areas, we also attempt to develop novel and safe immunotherapeutic approaches to specifically down-regulate platelet antibodies.
If we were to summarize the majority of the scientific literature regarding the immune pathogenesis of AITP, it might paint the following picture:




It appears that the major autoimmune stimuli initiating AITP occur at the APC-Th cell synapse. Collectively, these data support the need for clinical trials relating to T cell and APC modification in AITP(e.g. GPIIbIIIa peptide therapy etc.).

Our most recent studies suggest that platelets' express Toll-like receptors (particularly TLR4) and act as circulating sentinals to sence infections (Aslam et al Blood 2006). TLR expression on platelets also appears to significantly affect platelet destruction, especially when platelets are opsonized with anti-platelet antibodies (Semple et al Blood, 2007). These mechanisms may be the reason why thrombocytopenia worsens in some patients with AITP during infections and, alternatively, resolves in other patients with AITP who are treated with bacterial eradication therapy. This is summarized in the following diagram:


1. Immune dysregulation leads to the production of anti-platelet autoantibodies, RES phagocytosis and thrombocytopenia. 2. When some ITP patients become infected e.g with bacteria (containing LPS), their platelet destruction worsens and even more platelets are destroyed (counts drop further). If the infections is eradicated, the platelet destruction is relieved and platelet counts can rise.
On the other hand, for allogeneic platelet transfusions, our results in mice suggest the following immune mechanisms are involved:

Our current research efforts focus on the following projects:




If you would like more information on these subjects then feel free to email me at:
semplej@smh.toronto.on.ca


Please sign our Guestbook Guestbook by GuestWorld View the Guestbook


The following sections outline more details of the lab's activities

(INCLUDING): Post-doc, grad student and current employment information


Selected Publications:

Kjeldsen-Kragh J et al. Haematologica. 94(3):441-443, 2009.
Semple JW et al. Transfusion 48(9):1917-1924, 2008.
Aslam R et al. Transfusion 48(9):1778-1786, 2008. (Featured in an editorial)
Semple E et al. J Oral Maxillofac Surg. 66(4):632-638, 2008.
Ofosu F et al. Thromb Haemost 99(5):851-862, 2008. (Review)
Semple et al. Vox Sang 3(1):152-158, 2008. (Review)
Semple JW Blood 112(4): 927-928, 2008. (Inside Blood)
Semple JW. Thromb Haemost 99(2), 249, 2008. (Editorial)
Aslam R et al. Transfusion 47(11): 21612168, 2007.
Cognasse F et al. Current Immunol Rev 3:109-115, 2007 (Review)
Semple JW. Eur J Heamtol 79(4):279280, 2007 (Editorial)
Semple JW. Blood 110(8), 2784-2785, 2007, (Inside Blood)
Aslam R et al Transfusion 47:2161, 2007
Kjaersgaard M et al Blood 110:1359, 2007
Semple JW et al Blood 109:4803, 2007
Semple JW et al Drug Discovery Today 3:231, 2006
Aslam R et al Blood, 107:637,2006
Semple JW et al Sem Hematol 42:122, 2005
Sayeh E et al Blood 103:2705, 2004
Sayeh E et al Transfusion 44:1572, 2004
Coopamah MD et al Blood 102:2862, 2003
Coopamah MD et al Transfusion Medicine Rev 17:69, 2003
Semple JW et al J Pediatr Hematol Oncol 25 (Suppl 1), 11S, 2003
Semple JW et al Transfusion 42:958, 2002 (COVER ARTICLE - See above)
Semple JW et al Blood 100:1055, 2002
Semple JW et al Amer J Hematol 69:225, 2002


Post-Doctoral Fellows and Graduate Students:

My lab has an active training program for post-doctoral fellows and graduate students who are interested in pursuing studies related to transfusion immunology.

To apply for a post-doctoral position, please send your CV and a brief summary of research accomplishments to me at the above email address.

If you are interested in pursuing graduate studies in my lab (e.g. MSc or PhD), you need to be first accepted by the School of Graduate Studies at the University of Toronto. For more information about U of T. admission requirements, follow the following links:

Grad. studies in the Dept. of Pharmacology.
Grad. studies in the Institute for Medical Sciences.
Grad. studies in the Dept of Lab. Medicine and Pathobiol.


Announcements:


Eleventh European Symposium on Platelet and Granulocyte Immunology, Beaune, France, Sept,2010, Details to Follow



Selected papers from the lab...

Selected lectures from the lab...

Lab Photos.......

Other links of interest:

The PDSA: A support organization for people with ITP.
A UK based ITP support group.
Dr. Sandler's article on ITP.
The American autoimmune-related disease association.
The American Association of Blood Banks.
The American Society of Hematology.
An excellent site with many links to medicine.
Another excellent site with links to immunology.
Protein Reviews on the web.
Clinical trials listings by disease category.
Cytokines on the web.
Cangene: The makers of anti-D.
Canadian Institutes of Health Research.
National Institutes of Health.
Bayer Canada: Canada's leading supplier of IVIg.