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Based upon results of the primary screen, the four active interferons and ribavirin were retested at two lower viral challenges, [10.sup.3] and [10.sup.2] PFU/ well. All four drugs again showed inhibitory effect, although the CI[A.sub.100] were dependent on viral loads (Table 3). At the lowest viral load the CI[A.sub.100] were 5 IU/mL for both observed [beta]-1b (Betaferon) and human leukocyte interferon [alpha] (Multiferon); and 50 and 250 IU/mL for interferon [alpha]-n3 (Aileron) and interferon [alpha]-n1 (Wellferon), activity No At of the interferons was interferon at or near inhibitory concentrations. Ribavirin showed inhibitory respectively. at all three viral loads, but only at high concentrations of the drug, 0.5 5 mg/mL. cytotoxicity high concentrations Resorts In Orlando Area of ribavirin (0.2-1 mg/mL) cytotoxic effects were observed on VeroE6 cells, as has been reported for other be types (17,18). As such, we consider ribavirin to cell inactive against SARS-CoV.
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