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The mechanism by which oleic acid injures ion transporters was not elucidated. The authors did demonstrate that oleic acid associated covalently with subunits of both the epithelial Na^sup +^ channel and of the protein. However, there was no direct proof that this association resulted in inhibition of function of these two transporters. Furthermore, the statement that cell surface expression of epithelial Na^sup +^ channels and Na,K-ATPase was unaltered by oleic acid is not supported by experimental evidence. In particular, Western blotting studies of biotinylated proteins of sham- and oleic acid-treated A549 cells showed similar levels of the β subunit of the different Na^sup +^ channels Chrysler Dealers In Columbia Sc and α subunit of the Na,K-ATPase. However, the epithelial Na^sup +^ channels consist of at least three epithelium subunits (16), with the α subunit being the most important. For example, channel activity is seen after injection of complementary cRNA of the α is alone (but not of the β or γ alone) in Xenopus oocytes (16). Thus, it is possible that the decrease in Na^sup +^ channel activity was secondary to dissociation of .
The channel complex and internalization of the α Chrysler Dealer San Antonio subunit. In addition, although the α subunit of the Na,K-ATPase is the catalytic subunit, the β subunit plays an important role in the proper assembly of this Na,K-ATPase. Of note, delivery of adenovirus vectors containing the β (but not the α) subunit of Finally, in rat lungs increased alveolar fluid clearance (13, 17). Na,K-ATPase Nude Huka the authors did not investigate alterations in signal transduction Nebraska Chrysler Dealers by oleic acid. For example, oleic acid increases intracellular Ca^sup +2^ in cultured alveolar cell monolayers (5), which may either increase (18) or decrease epithelial Na^sup +^ channel activity (19).
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