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Jim Campanella's Homepage

James Campanella

Assistant Professor in The Biological Sciences

Department of Biology and Molecular Biology

Ph.D., Case Western Reserve University

M.S., University of Chicago

B.A., University of Chicago


RESEARCH AND TEACHING INTERESTS:

I work in plant genetics and plant phylogenetics. My particular interest lies in the crucifer Arabidopsis thaliana. We have a variety of projects going on in my laboratory at the moment.

1) Our Main project is the study of the molecular evolution and intraspecific/interspecific phylogeny of Arabidopsis thaliana. Arabidopsis is a species that is grows over most of the world-- Europe, North America, Africa, Asia, and Australia. We are interested in how the various populations that have been isolated over evolutionary time have changed and altered from one another both physically and genetically. We study differences in DNA polymorphic markers between the various populations and use those differences to construct statistical models that help us predict just how much isolated groups have changed from one another. This can help us to understand how speciation occurs-- speciation is the process where two isolated populations of the same species become two separate species.

2) We are in the process of cloning a mutant gene from Arabidopsis thaliana called tu8. The tu8 mutation is pleiotrophic, meaning that it is a single locus mutation but causes multiple phenotypic alterations in the mutant. Although the mutation was originally isolated due to a lesion in a biochemical pathway, the mutation also causes the affected plant to be dwarfed, hyper-sensitive to heat, lose its basal leaves at an early age, and flower earlier than wild-type plants. We have mapped this mutation to chromosome 5 of Arabidopsis, and now we are using positional cloning techniques to isolate the gene.

3) Finally, we are interested in the storage and utilization of the plant hormone Indole Acetic Acid (IAA). My lab is presently cloning homologs from Arabidopsis suecica of an enzyme utilized in activation of IAA from it storage form in plants.

In addition to the regular courses that I teach I also mentor undergraduate students in independent research as they aid me in these various research projects. Students learn the scientific method, how to think independently, how to analyze data, how to properly prepare scientific notebooks, and how to present their data to others. Of course, all this work is in addition to learning different laboratory methods in molecular genetics and plant biology.

Teaching Information:

* Genetics, Biology 380

* Medical Genetics, Biology 480

* Cell Biology, Biology 230


Campanella Lab Bibliography

Long-term field storage and molecular viability of tissue samples from buccal swabbing of sunfish for DNA extraction. 2005. James Campanella and John Smalley. Frontiers in Zoology. (submitted).

Molecular Characterization of the auxin amidohydrolase family in Medicago truncatula. 2005. James J. Campanella, Stephanie M. Smith, Shiri Wexler, LaShire Hull and Jutta Ludwig-Müller. Plant Physiology. (submitted).

Utilization of Sea Scallop (Placopecten magellanicus Gmelin) microsatellite markers for phylogenetic applications in bay scallops (Argopecten irradians Lamarck). 2005. James J. Campanella, Liliana E.J. Kim, Paul A.X. Bologna. Journal of Shellfish Research. (submitted).

Utilizing buccal swabbing for collection and extraction of high-quality, newt (Notophthalmus) DNA for use in phylogenetic analyses. 2005. James Campanella and John Smalley. In Vivo. (in press).

A Phylogenomic study of the alphavirus family employing whole genome comparison. 2005. Aimee Luers, Sandra Adams, John Smalley, and James Campanella. Comparative and Functional Genomics. (in press).

A simple method for collection and extraction of high quality, sunfish (Lepomis) DNA for use in polymerase chain reaction analysis. 2005. John Smalley and James Campanella. Biotechniques. 38(2):190-191.

A novel auxin conjugate hydrolase from Triticum aestivum with substrate specificity for longer side chain auxin amide conjugates. 2004. James J. Campanella, Jutta Ludwig-Mueller, Adebanke Olajide, and Volker Magnus. Plant Physiology. 135:2230-2240.

Microarray analysis in Brassicaceae: hybridization of Arabidopsis thaliana cDNA arrays with cDNA from a radiation-induced plant tumor and normal plant tissues. 2004. James J. Campanella, Chunguang Du, Quinn Vega, Olumbusola Gomes and William Graff. The Journal of Young Investigators. (in press).

A Molecular Polymorphic Analysis of Brassicaceae Outgroups for Use in Phylogeny Studies with Arabidopsis Thaliana. 2004. James J. Campanella, Vishnu Singh, Nicholas Kumburis, John Smalley and Zankhana Thakkar. In Vivo. 25(3):14-25.

A Molecular Phylogenomic Analysis of the ILR1-like Family of IAA Amidohydrolase Genes. 2003. James J. Campanella, Daniel Larko and John Smalley. Comparative and Functional Genomics . 4(6):584-600.

ILR1 and sILR1 IAA amidohydrolase homologs differ in expression pattern and substrate specificity. 2003. James J. Campanella, Jutta Ludwig-Mueller, Vinela Bakllamaja, Vipul Sharma and Ania Cartier. Plant Growth Regulation. 41(3): 215-223.

Expression analysis of Amidohydrolase Homologs from Arabidopsis thaliana and Arabidopsis suecica. 2003. James Campanella and Vinela Bakllamaja. The Journal of Young Investigators. 8:1.

MatGAT: An application that generates similarity/identity matrices using protein or DNA sequences. 2003. James J. Campanella, Ledion Bitincka and John Smalley. BMC Bioinformatics. 4:29.

Molecular Evidence Supporting the Asian Origin Hypothesis of Arabidopsis. 2003. James Campanella, Vishnu Singh, Nicholas Kumburis, Karen Vargas, and Alexander Rios. In Vivo. 25(1): 4-11.

Employing the GenBank and TIGR Databases for Phylogenetic Analysis and Gene Functional Predictions. 2003. James J. Campanella, Daniel Larko and John Smalley. In Vivo. 24(2): 4-11.

Patterns of Cytoplasmic Variation in Arabidopsis thaliana (Brassicaceae) revealed by polymorphic chloroplast microsatellites. 2003. James Provan and James Campanella. Systematic Botany. 28(3): 578-583.

Isolation of an Amidohydrolase Homolog from Arabidopsis suecica. 2003. James J. Campanella, Vinela Bakllamaja, Shahin Shahtaheri, Michael Vomacka, Jason Herron, Megan Patterson, and Tracie Restieri. Plant Growth Regulation. 39:175-181 .

The Intraspecific Phylogenetics of Arabidopsis thaliana in World-Wide Populations. Christine Vander Zwan, Seth Brodie and James J. Campanella. 2000. Systematic Botany, 25(1):47-59.

Mapping of icr Genes Reveals That tt6 Should Be Placed on Chromosome 5. James J. Campanella, Weiqin Dong and Christopher D. Town. 1997., Weeds World 4(i):16-17.

Isolation and Characterization of Two Arabidopsis Mutants with Increased Resistance to Growth Inhibition by Indoleacetic Acid-Amino Acid Conjugates. J.J. Campanella, J. Ludwig-Mueller and C.D. Town. 1996., Plant Physiology, 112:735-745.

Use of Radiation-Induced Tumors to Study Growth Control in Arabidopsis. C.D. Town, B.R. Campell, S.M. Persinger, and J.J. Campanella. 1994., In Vitro Cell Dev.Biol. 30P:89-95.

Interactions of Microbotryum Violaceum (Ustilago violacea) with Its Host Plant Silene alba. M. Ruddat, J. Kokontis, L. Birch, E.D. Garber, K.S. Chiang, J. Campanella and H. Dai. 1991., Plant Science 80 (1-2):157-166.

ENCYCLOPEDIA ARTICLES:

Plant Molecular Systematics. James Campanella. 2002. The Encyclopedia of Plant Life. (Ed. Bryan Ness). Salem Press, California. Pg. 686-688.

Genetics: Post-Mendelian. James Campanella. 2002. The Encyclopedia of Plant Life. (Ed. Bryan Ness). Salem Press, California. Pg. 505-509.

The Biomedical Ethics of Organ Transplantation. James Campanella. 2001. In: MaGill’s Medical Guide. (Editor: Tracy Irons-Georges ). 2nd Edition. Salem Press, California. pg. 2304-2309.

An Update on Microbial Drug Resistance. James Campanella. 2001. In: MaGill’s Medical Guide. (Editor: Tracy Irons-Georges ). 2nd Edition. Salem Press, California. Pg. 661-665.

Recent Advances in Cancer and Treatment. James Campanella. 2001. In: MaGill’s Medical Guide. (Editor: Tracy Irons-Georges ). 2nd Edition. Salem Press, California. Pg.338-343.

Multiple Sclerosis: an Update on Etiology and Treatments. James Campanella. 2000. In: Aging (Editor: Pamela Roberts) pg. 544-548. Salem Press, California.

Advances in Longevity Research. James Campanella. 2000. In: Aging (Editor: Pamela Roberts) pg. 475-479. Salem Press, California.

Cloning: Ethical Issues. James Campanella. 1998. in: The Encyclopedia of Genetics. (Editor: Jeffery Knight) pg. 116-119. Salem Press, California.

Biopesticides. James Campanella. 1998. in: The Encyclopedia of Genetics. (Editor: Jeffery Knight) pg.54-57. Salem Press, California.

DNA Isolation. James Campanella. 1998. in: The Encyclopedia of Genetics.(Editor: Jeffery Knight) pg. 152-155. Salem Press, California.


Contact information :
Professor James Campanella
Department of Biology and Molecular Biology
Science Hall, Office: Room 118, Laboratory: Room 302
1 Normal Avenue
Montclair State University
Upper Montclair, New Jersey 07043

Telephone: (973) 655-4097
FAX: (419) 791-9834
Email: campanellj@mail.montclair.edu
URL: http://www.angelfire.com/nj2/arabidopsis

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