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Manji- Master swordsman who assassinated his own corrupt lord. Granted immortality by 800-year-old Yaobikuni, Manji may only find the release of death after killing a thousand evil men.

Rin- Daughter of Asano, head of the Mutenichi-ryo dojo. Rin's parents were murdered by Anotsu and his followers, the Itto-Ryu. Now Rin has enlisted Manji's aid to avenge her parents' death.

Anotsu- Head of the Itto-Ryu, Kagehisa proves an elusive victim. A first-grade swordsman, he has little compassion and even littler patience. He sees Rin and Manji as gnats waiting to be exterminated.