MICROBIOlOGY LAB SCHEDULE

Instructions: Except as noted, the following schedule of laboratory exercises can be found in the Lab Manual : "Selected Exercises from Microbes in Action by Seeley and Van Demark.

Students should read each exercise before coming to the lab. There may be a pre-lab quiz before the lab. The lab schedule is tentative and may be modified

Laboratory Exercises will be done in the following order:

Lab #                           Exercise

Date

9-5

1. Experiments 5 and 6 (Asceptic Transfer of Bacteria into broth and agar slants)

1. Use of the Microscope

1. Experiment 1 (Examination of stained cells under oil immersion).

9-9
2. Experiment 10: Direct Staining with Basic Dyes 

2. Handout will be provided for Negative Staining.

(students will stain cultures they prepared in experiments 5 and 6)

9-11

3. Experiment 11: The Gram Stain (Differentiating between bacterial types)

For help interpreting Gram Stains Go to:

http://www.meddean.luc.edu/lumen/DeptWebs/microbio/med/gram/exam-gs.htm  

3. Experiment 12:(Only Endospore Stain part).

9-16

4. Experiment 27: Carbohydrate Degradation,

https://www.angelfire.com/mi/nccc/sugar.html Click here to see lab results from last years Carbohydrate fermentation tests.

4. Handout on Lipid Hydrolysis

9-18
5. Experiment 28: Degradation of Proteins and Amino Acids

https://www.angelfire.com/mi/nccc/milk.html Click here to see an example of milk reactions from various bacteria

9-23

6. Experiment 17: Osmotic pressure lab handout

https://www.angelfire.com/mi/nccc/product.html Click here to see the effect of salt on bacterial growth as seen in last years lab.

6. Experiment 30: Catalase Activity -Parts A and B.

9-25

7. Experiment 7: Separating Mixed Cultures into Pure Cultures.

https://www.angelfire.com/mi/nccc/Separate.html Click on this line to view lab results from last year's classes

7. Experiment 14: Quantitative Plating Methods: Determination of Microbial Numbers: Pour Plate method. (p. 84 in Manual. Read preceding pages).

9-30

8. Experiment 19 Energy Sources and Buffers

8.      Experiment 32 (modified) Selective plating EMB

https://www.angelfire.com/mi/nccc/labwork.html Click here to learn how to tell the difference between E. coli and Enterobacter aerogenes in the lab

8. Also Acid-fast and capsule staining

10-2
9. Experiments 17 Effects of Temperature

9. Experiments 18 Effects of Heat

9. Experiments 21 and 22: Oxygen and Microbial Growth and Culture of Anaerobic Bacteria

10-7

10. Experiment 9(demonstration only)

10. Review unknown procedure

10. Notebooks Due

10-9

11. Chapter 11/Distribute Unknowns.

10-14

12. Chapter 12/Unknowns.

10-16

13.  Experiment 23 and Fingerprint plates(handout)/Unknowns

10-21

14. Unknowns.

10-23

15. Experiment 51 Antibiotics/Unknowns.

10-28

16 Unknowns write-up  due/ UV light experiment Handout

16. LAb test #1/Notebooks due

10-30

17. Transformation Experiment (Handout)

11-4

18. Plasmid Prep(Handout)

11-6

19. Plasmid Identification(Handout)

11-11

20. Plasmid Identification(continued).

20.  HIV Simulation Handout.

11-13

21. Discuss Group Lab projects

11-18

22. An Antibody ID Test: Staph Latex Test .

22. ELISA (Handout)

11-20

23. Experiment 40 Coliform Standard Analysis of Local Water

11-25

24. Experiment 40 continued.

12-2

25. Lab Test #2/Notebooks due

12-4

24-27 Work on Lab Projects

12-9

25-27. Lecture Presentations(first 50 minutes)

12-11

28.  Present Lab Projects

Grading:

Notebooks/Handouts:   30%

Pre-lab quizzes:            10%

Tests:                           20%

Unknowns:                   20%

Group Projects:            20%

Microbiology Lab Protocol

You will be provided with a lab coat and will need to use latex gloves and safety glasses in the lab.

You will not eat or drink or smoke in the lab.

You will wash your hands before and after each lab

You will wash down all work areas with disinfectant prior to and after each lab.