Microbiology Lab Protocol and Schedule

You will be provided with a lab coat and will need to use latex gloves and safety glasses in the lab.

You will not eat or drink or smoke in the lab.

You will wash your hands before and after each lab

You will wash down all work areas with disinfectant prior to and after each lab.

Laboratory Manual: Selected Exercises from Microbes in Action by Seeley and Van deMark.

MICROBIOlOGY LAB SCHEDULE

Instructions: Except for one or two labs the following schedule of laboratory exercises can be found in the Lab Manual : "Selected Exercises from Microbes in Action by Seeley and Van Demark.

Students should read each exercise before coming to the lab. There may be a prelab quiz before the lab. The lab schedule is tentative and may be modified

Laboratory Exercises will be done in the following order:

1. Experiments 5 and 6 (Asceptic Transfer of Bacteria into broth and agar slants)

Click here to understand how bacterial colonies with pigments can be told apart.

1. Use of the Microscope

Click here to understand the history of the microscope

1. Experiment 1 (Examination of stained cells under oil immersion).

2. Handout will be provided for Negative Staining.

3. Experiment 11: The Gram Stain (Differentiating between bacterial types)

  Click for help with EXAMINATION AND INTERPRETATION Of Gram Stains                                 

3. Experiment 12:(Only Endospore Stain part).

4. Experiment 27: Carbohydrate Degredation

Click here to show lab results from last years Carbohydrate fermentation tests.

4. Handout on Lipid Hydrolysis

5. Experiment 28 Degredation of Proteins and Amino Acids

Click here to see an example of milk reactions from various bacteria