Site hosted by Build your free website today!


What ELISPOT assays tell you

ELISPOT assays are used to detect very small amounts of cytokines secreted by T lymphocytes in culture. It requires concentrating the cytokine molecules in small areas of a culture well by having them bind to anti-cytokine antibodies coating the plate.  The cells are then removed, after which an enzyme-labeled anti-cytokine and a chromogenic substrate for the enzyme are used to graphically detect the bound cytokines.

Through this technique, one can count the number of cytokine-secreting cells in each well.

Additional work to be added sometime...