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~Stem Cells~
:: What are they? ::
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:: Stem Cell Assays ::
~Cloning~
:: How it works- Somatic Cell Nuclear Transfer ::
:: Uses ::
:: Problems/Issues ::
"We are not static beings. Our tissues turn over constantly and there must be cells that remember the form" [of the original organ].
--James L. Sherley, Assoc. Professor of Biological Engineering in MIT's Center for Environmental Health Science and Center for Cancer Research
SCNT has been shown to be a promising way of deriving pluripotent stem cells. It involves fusing a somatic cell with an enucleated oocyte, where the nucleus of the somatic cell provides the genetic information.
Steps:
1) Extract somatic cell from the G1 phase.
2) Remove DNA from an egg.
3) Insert DNA from the donor cell into the egg.
4) Stimulate the mitotic inducers in the cytoplasm.
5) Transplant the early embryo into the surrogate mother.
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