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RTPrimerDB Real Time PCR Primer and Probe Database

Quick Search / Advanced Search Gene Substring Exact phrase Organism   RTPrimerDB ID All Homo sapiens Mus musculus Rattus norvegicus Drosophila melanogaster Danio rerio Bos taurus Caenorhabditis elegans Human immunodeficiency virus 1 Introduction This is a public database holding real time PCR primers and probes for popular  Beacons) to prevent time-consuming primer design and experimental optimisation. We strongly encourage researchers to submit their validated primer and probe sequences. One can query the database using the official gene name, LocusLink or SNP identifier, or oligonucleotide sequence. Different options make it possible to restrict a query to one application (gene expression quantification/detection, DNA copy number quantification (e.g. oncogene amplification), mutation analysis, SNP detection, fusion gene quantification/detection), organism (human, fruit fly, mouse, rat, zebrafish, cow, nematode or HIV-1) or detection chemistry. Data submission is allowed after free registration where you obtain a login name and password. Currently, 718 primer sets are available, submitted by 121 people. What's new July 2002: database goes online August 2002: BLAST feature for primer/probe sequences September 2002: possibility to submit records for fusion gene detection/quantification in malignancies October 2002: database download feature available November 2002: restyling of the site in a frameless version possibility to directly link to an RTPrimerDB record new organism added: Bos taurus (cow) December 2002: new organism added: Caenorhabditis elegans (nematode) new organism added: Human immunodeficiency virus 1 (HIV-1) March 2003: update of FAQ's query and update feature of submitted primer/probe sets Publications PATTYN, F., SPELEMAN, F., DE PAEPE A. & VANDESOMPELE, J. (2003). RTPrimerDB: the Real-Time PCR primer and probe database. Nucleic Acids Research, 31(1): 122-123. [Download]