Dr. Thomas Smith
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Apyrases

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Dr. Thomas M. Smith

Dr. Thomas M. Smith

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Journal Articles

Dai, J., Liu, J., Deng, Y., Smith, T.M., and Lu, M.  2004. Structure and protein design of a human platelet function inhibitor. Cell.  116(5): 649-659.

Smith, T.M., Hicks-Berger, C., Kim, S. and Kirley, T.L.  2002. Cloning, expression, and characterization of a soluble calcium-activated nucleotidase, a human enzyme belonging to a new family of extracellular nucleotidases. Archives of Biochemistry and Biophysics.  406(1): 105-115.

Smith, T.M. and Emmeluth, D.  2002. Introducing bioinformatics into the biology curriculum: exploring the National Center for Biotechnology Information. American Biology Teacher.  64(2): 93-99.

Hicks-Berger, C.A., Yang, F., Smith, T.M. and Kirley, T.L.  2001. The importance of histidine residues in human ecto-nucleoside triphosphate diphosphohydrolase-3 as determined by site-directed mutagenesis. Biochimica et Biophysica Acta.  1547(1): 72-81.

Yang, F., Hicks-Berger, C.A., Smith, T.M. and Kirley, T.L.  2001. Site-directed mutagenesis of human nucleoside triphosphate diphosphohydrolase 3: the importance of residues in the apyrase conserved regions. Biochemistry.  40(13): 3943-50.

Kirley, T.L., Gerber, L.K., and Smith, T.M.  1999. Expression and characterization of chicken muscle ecto-ATPase in mammalian COS cells. IUBMB Life.  48(1): 67-72.

Smith, T.M., Lewis Carl, S.A., and Kirley, T.L.  1999. Mutagenesis of two conserved tryptophan residues of the E-type ATPases: Inactivation and conversion of an ecto-apyrase to an ecto-NTPase. Biochemistry.  38 (18): 5849-5857.

Smith, T.M., and Kirley, T.L.  1999. Glycosylation is essential for functional expression of a human brain ecto-apyrase. Biochemistry.  38 (5): 1509-1516.

Smith, T.M., and Kirley, T.L.  1999. Site-directed mutagenesis of a human brain ecto-apyrase: Evidence that the E-type ATPases are members of the Actin/Heat Shock 70/Sugar Kinase superfamily. Biochemistry.  38 (1): 321-328.

Smith, T.M., and Kirley, T.L.  1998. Cloning, sequencing, and expression of a human brain ecto-apyrase related to both the ecto-ATPases and CD39 ecto-apyrasesBiochimica et Biophysica Acta. 1386 (1): 65-78.

Smith, T.M., Lewis Carl, S.A., and Kirley, T.L.  1998. Immunological detection of ecto-ATPase in chicken and rat tissues: characterization, distribution, and a cautionary noteBiochemistry and Molecular Biology International 45: 1057-1066.

Lewis Carl, S.A., Smith, T.M., and Kirley, T.L.  1998. Cross-linking induces homodimer formation and inhibits enzymatic activity of chicken stomach ecto-apyraseBiochemistry and Molecular Biology International.  44: 463-470.

Smith, T., Kirley, T., and Hennessey, T.  1997. A soluble ecto-ATPase from Tetrahymena thermophila: Purification and similarity to the membrane-bound ecto-ATPase of smooth muscleArchives of Biochemistry and Biophysics.  337: 351-359.

Antson, A., Otridge, J., Brzozowski, A., Dodson, E., Dodson, G., Wilson, K., Smith, T., Yang, M., Kurecki, T., and Gollnick, P.  1995. The structure of trp RNA-binding attenuation protein. Nature.  374: 693-700.

Smith, T., and Hennessey, T.  1993. Body plasma membrane vesicles from Paramecium contain a vanadate-sensitive calcium ATPaseAnalytical Biochemistry.  210: 299-308.
 

Text Chapters

Smith, T.M., and Kirley, T.L.  2000.  "Expression, characterization, and site-directed mutagenesis of a human E-type ATPase."  in Ecto-ATPases and Related Ectonucleotidases (Vanduffel, L., and Lemmens, R., Eds.). 90-105.  Shaker Publishing, Maastricht, The Netherlands.

Smith, T., Kim, M., Kirley, T., and Hennessey, T.  1997.  "Ecto-ATPase of Tetrahymena: Role in Purinergic Responses and Purification of a Soluble Form."  in Ecto-ATPases: Recent Progress on Structure and Function (Plesner, L., Kirley, T., and Knowles, A., Eds.). 135-142.  Plenum Press, New York.
 

Abstracts

Smith, T.M., Kirley, T.L., and Hennessey, T.M.  1996.  Purification and characterization of a soluble ecto-ATPase from Tetrahymena thermophilaMolecular Biology of the Cell.  7: 435a.

Smith, T.M., and Hennessey, T.M.  1995.  Ecto-ATPase of Tetrahymena: Induction of activity by sodium and release of a soluble form.  Molecular Biology of the Cell.  6: 180a

Hennessey, T., Smith, T., Corcoran, G., and Gielow, M.  1991.  Body plasma membrane vesicles from Paramecium. The Journal of Cell Biology.  115: 85a.


Publications of Dr. Thomas M. Smith

Bioinformatics

NCBI homepage

See my "Bioinformatics in the Classroom" modules featured on the National Center for Biotechnology Information homepage.

Apyrases

Apyrases

See my chapter in the text that Barnes&Noble.com states, "demonstrated the important roles of ecto-ATPases in thromboregulation, and transplant rejection..."

Modified 07/15/2004

© 2004 Thomas Smith